Evidence base

Studies on Ashwagandha (KSM-66)

Auto-loaded from PubMed — grouped by outcome. Each entry links to the original source.

1,975 studies total·Last updated: May 27, 2026Open on PubMed

Sleep · 25

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  1. 01
    Dietary Protocols to Promote and Improve Restful Sleep: A Narrative Review
    Conti F · Nutr Rev · 2026
  2. 02
    Efficacy and safety of Ashwagandha root extract sustained-release (AshwaSR) capsules in healthy adult, stressed subjects: A randomized, double-blind, placebo-controlled, parallel-group, 3-arm clinical trial
    Thanawala S, Shah R, Alluri KV et al. · Medicine (Baltimore) · 2026RCT
  3. 03
    Effects of multi-herb and ashwagandha root formulas on stress modulation: a randomized, double-blind, placebo-controlled clinical study
    McKinney E, Stewart J, Kewalramani R et al. · Trials · 2026RCT
  4. 04
    A New Ashwagandha Formulation (Zenroot™) Alleviates Stress and Anxiety Symptoms While Improving Mood and Sleep Quality: A Randomized, Double-Blind, Placebo-Controlled Clinical Study
    Mahadevan M, Gopukumar K, Gupta R et al. · Adv Ther · 2025RCT
  5. 05
    Integrative sleep management: from molecular pathways to conventional and herbal treatments
    Ebrahim YM, Sadek MA, Sabry MO et al. · Naunyn Schmiedebergs Arch Pharmacol · 2025
  6. 06
    Ashwagandha (Withania somnifera) and Its Effects on Well-Being-A Review
    Wiciński M, Fajkiel-Madajczyk A, Sławatycki J et al. · Nutrients · 2025
  7. 07
    A Novel Ashwagandha (Withania somnifera) Formulation Mitigates Sleep Deprivation-Induced Cognitive Impairment and Oxidative Stress in a Rat Model
    Er B, Ozmen B, Sahin E et al. · Biomolecules · 2025
  8. 08
    Safety and efficacy of Withania somnifera for anxiety and insomnia: Systematic review and meta-analysis
    Fatima K, Malik J, Muskan F et al. · Hum Psychopharmacol · 2024SR
  9. 09
    Sleep-promoting activity of amylase-treated Ashwagandha (Withania somnifera L. Dunal) root extract via GABA receptors
    Park CW, Hong KB, Suh HJ et al. · J Food Drug Anal · 2023
  10. 10
    Complementary and Integrative Approaches to Sleep Disorders in Children
    Deshpande SN, Simkin DR · Child Adolesc Psychiatr Clin N Am · 2023
  11. 11
    Ashwagandha Modulates Stress, Sleep Dynamics, and Mental Clarity
    Zemel PhD MB · J Med Food · 2022
  12. 12
    The Impact of Ashwagandha on Stress, Sleep Quality, and Food Cravings in College Students: Quantitative Analysis of a Double-Blind Randomized Control Trial
    O'Connor J, Lindsay K, Baker C et al. · J Med Food · 2022RCT
  13. 13
    The Perceived Impact of Ashwagandha on Stress, Sleep Quality, Energy, and Mental Clarity for College Students: Qualitative Analysis of a Double-Blind Randomized Control Trial
    Baker C, Kirby JB, O'Connor J et al. · J Med Food · 2022RCT
  14. 14
    Effect of Ashwagandha (Withania somnifera) extract on sleep: A systematic review and meta-analysis
    Cheah KL, Norhayati MN, Husniati Yaacob L et al. · PLoS One · 2021Meta
  15. 15
    Medicinal Plants for Insomnia Related to Anxiety: An Updated Review
    Borrás S, Martínez-Solís I, Ríos JL · Planta Med · 2021
  16. 16
    Clinical evaluation of the pharmacological impact of ashwagandha root extract on sleep in healthy volunteers and insomnia patients: A double-blind, randomized, parallel-group, placebo-controlled study
    Langade D, Thakare V, Kanchi S et al. · J Ethnopharmacol · 2021RCT
  17. 17
    Effects of Withania somnifera (Ashwagandha) on Stress and the Stress- Related Neuropsychiatric Disorders Anxiety, Depression, and Insomnia
    Speers AB, Cabey KA, Soumyanath A et al. · Curr Neuropharmacol · 2021
  18. 18
    A randomized, double blind, placebo controlled study to evaluate the effects of ashwagandha (Withania somnifera) extract on sleep quality in healthy adults
    Deshpande A, Irani N, Balkrishnan R et al. · Sleep Med · 2020RCT
  19. 19
    Screening of sleep assisting drug candidates with a Drosophila model
    Wang YY, Ma WW, Peng IF · PLoS One · 2020
  20. 20
    Study protocol and rationale for a prospective, randomized, double-blind, placebo-controlled study to evaluate the effects of Ashwagandha (Withania somnifera) extract on nonrestorative sleep
    Deshpande A, Irani N, Balakrishnan R · Medicine (Baltimore) · 2018
  21. 21
    Aqueous Leaf Extract of Withania somnifera as a Potential Neuroprotective Agent in Sleep-deprived Rats: a Mechanistic Study
    Manchanda S, Mishra R, Singh R et al. · Mol Neurobiol · 2017
  22. 22
    Withania somnifera as a potential anxiolytic and immunomodulatory agent in acute sleep deprived female Wistar rats
    Kaur T, Singh H, Mishra R et al. · Mol Cell Biochem · 2017
  23. 23
    Triethylene glycol, an active component of Ashwagandha (Withania somnifera) leaves, is responsible for sleep induction
    Kaushik MK, Kaul SC, Wadhwa R et al. · PLoS One · 2017
  24. 24
    Protective effect of Withania somnifera Dunal on the behavioral and biochemical alterations in sleep-disturbed mice (Grid over water suspended method)
    Kumar A, Kalonia H · Indian J Exp Biol · 2007
  25. 25
    Studies on Withania ashwagandha Kaul. VI. The effect of the alkaloidal fractions (acetone, alcohol and water soluble) on the central nervous system
    Prasad S, Malhotra CL · Indian J Physiol Pharmacol · 1968

Stress & anxiety · 51

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  1. 01
    Safety and efficacy of ashwagen (a standardized withania somnifera extract) in stress and anxiety with hypertension and associated cardiometabolic risk factors: a randomized, placebo-controlled trial
    Pattojoshi A, Kumar Patra S, Idrees SA et al. · Stress · 2026RCT
  2. 02
    Effects of ashwagandha (Withania somnifera) on mental health in adults: A systematic review and dose-response meta-analysis of randomized controlled trials
    Alsanie SA, Alhodieb FS, Askarpour M · Complement Ther Med · 2026SR
  3. 03
    Clinical evidence for the adaptogenic effects of Withania somnifera and Rhodiola rosea - A systematic review with molecular interpretation of psychometric outcomes
    Łuszczak J, Kocki J · Ann Agric Environ Med · 2026SR
  4. 04
    Efficacy and safety of Ashwagandha root extract sustained-release (AshwaSR) capsules in healthy adult, stressed subjects: A randomized, double-blind, placebo-controlled, parallel-group, 3-arm clinical trial
    Thanawala S, Shah R, Alluri KV et al. · Medicine (Baltimore) · 2026RCT
  5. 05
    Mouse neuronal dendritic complexity and resilience to stress-induced depression in Drosophila melanogaster are enhanced by Withania somnifera alkaloids
    Dutta R, Holvoet H, Marney L et al. · J Ethnopharmacol · 2026
  6. 06
    Effects of multi-herb and ashwagandha root formulas on stress modulation: a randomized, double-blind, placebo-controlled clinical study
    McKinney E, Stewart J, Kewalramani R et al. · Trials · 2026RCT
  7. 07
    A proprietary herbal extract of ashwagandha root for stress and anxiety in healthy adults: a randomized, double-blind, three-arm, placebo-controlled efficacy and safety study
    West RE, Biswas A, Rao R et al. · J Med Life · 2026RCT
  8. 08
    Antidepressant-like effects of Ashwagandha (Withania Somnifera) on chronic unpredictable mild stress-induced depression in adolescent rats
    Gokdemir GS, Seker U, Baksi N et al. · Psychopharmacology (Berl) · 2026
  9. 09
    Efficacy and safety of Ashwagandha (Withania somnifera) root extract on stress and weight management in adults: a prospective, randomized, double-blind, placebo-controlled study
    Pakhale K, Pakhale R, Srivathsan M et al. · J Med Life · 2025RCT
  10. 10
    Dual impact of Ashwagandha: Significant cortisol reduction but no effects on perceived stress - A systematic review and meta-analysis
    Albalawi AA · Nutr Health · 2025SR
  11. 11
    A New Ashwagandha Formulation (Zenroot™) Alleviates Stress and Anxiety Symptoms While Improving Mood and Sleep Quality: A Randomized, Double-Blind, Placebo-Controlled Clinical Study
    Mahadevan M, Gopukumar K, Gupta R et al. · Adv Ther · 2025RCT
  12. 12
    Attenuation Effect of Withania somnifera Extract on Restraint Stress-Induced Anxiety-like Behavior and Hippocampal Alterations in Mice
    Lee K, Lee D, Kim JY et al. · Int J Mol Sci · 2025
  13. 13
    Ashwagandha (Withania somnifera) and Its Effects on Well-Being-A Review
    Wiciński M, Fajkiel-Madajczyk A, Sławatycki J et al. · Nutrients · 2025
  14. 14
    Ashwagandha (Withania somnifera (L.) dunal) root extract containing withanolide a alleviates depression-like behavior in mice by enhancing the brain-derived neurotrophic factor pathway under unexpected chronic mild stress
    Kim H, Choi HS, Han K et al. · J Ethnopharmacol · 2025
  15. 15
    Investigating the Impact of Ashwagandha and Meditation on Stress Induced Obesogenic Eating Behaviours
    Quinones D, Barrow M, Seidler K · J Am Nutr Assoc · 2025
  16. 16
    Effectiveness of a Saffron and Withania Supplement on Mood in Women With Mild-to-Moderate Anxiety During the COVID-19 Lockdown
    Pages-García C, De Almagro MC, Ruiz-Moreno J et al. · Depress Anxiety · 2024
  17. 17
    Effects of Ashwagandha (Withania Somnifera) on stress and anxiety: A systematic review and meta-analysis
    Arumugam V, Vijayakumar V, Balakrishnan A et al. · Explore (NY) · 2024SR
  18. 18
    Safety and efficacy of Withania somnifera for anxiety and insomnia: Systematic review and meta-analysis
    Fatima K, Malik J, Muskan F et al. · Hum Psychopharmacol · 2024SR
  19. 19
    Modulation by Withania somnifera of stress-induced anxiogenesis and airway inflammation in rats
    Rehman S, Naqvi M, Ali NH et al. · J Complement Integr Med · 2024
  20. 20
    A Standardized Withania somnifera (Linn.) Root Extract with Piperine Alleviates the Symptoms of Anxiety and Depression by Increasing Serotonin Levels: A Double-Blind, Randomized, Placebo-Controlled Study
    Majeed M, Nagabhushanam K, Murali A et al. · J Integr Complement Med · 2024RCT
  21. 21
    Effects of Withania somnifera Extract in Chronically Stressed Adults: A Randomized Controlled Trial
    Pandit S, Srivastav AK, Sur TK et al. · Nutrients · 2024RCT
  22. 22
    A standardized Ashwagandha root extract alleviates stress, anxiety, and improves quality of life in healthy adults by modulating stress hormones: Results from a randomized, double-blind, placebo-controlled study
    Majeed M, Nagabhushanam K, Mundkur L · Medicine (Baltimore) · 2023RCT
  23. 23
    Ashwagandha: An Effective Phytomedicine for Reducing Stress and Anxiety
    Ross SM · Holist Nurs Pract · 2023
  24. 24
    Does Ashwagandha supplementation have a beneficial effect on the management of anxiety and stress? A systematic review and meta-analysis of randomized controlled trials
    Akhgarjand C, Asoudeh F, Bagheri A et al. · Phytother Res · 2022Meta
  25. 25
    Clinician guidelines for the treatment of psychiatric disorders with nutraceuticals and phytoceuticals: The World Federation of Societies of Biological Psychiatry (WFSBP) and Canadian Network for Mood and Anxiety Treatments (CANMAT) Taskforce
    Sarris J, Ravindran A, Yatham LN et al. · World J Biol Psychiatry · 2022Meta

Hormones · 79

Open on PubMed
  1. 01
    Safety and efficacy of ashwagen (a standardized withania somnifera extract) in stress and anxiety with hypertension and associated cardiometabolic risk factors: a randomized, placebo-controlled trial
    Pattojoshi A, Kumar Patra S, Idrees SA et al. · Stress · 2026RCT
  2. 02
    Dietary Protocols to Promote and Improve Restful Sleep: A Narrative Review
    Conti F · Nutr Rev · 2026
  3. 03
    Efficacy and safety of Ashwagandha root extract sustained-release (AshwaSR) capsules in healthy adult, stressed subjects: A randomized, double-blind, placebo-controlled, parallel-group, 3-arm clinical trial
    Thanawala S, Shah R, Alluri KV et al. · Medicine (Baltimore) · 2026RCT
  4. 04
    Ashwagandha Root Extract Stabilises Physiological Stress Responses in Male and Female Team Sports Athletes During Pre-Season Training
    Coope OC, Otaegui E, Suárez M et al. · Nutrients · 2026RCT
  5. 05
    A proprietary herbal extract of ashwagandha root for stress and anxiety in healthy adults: a randomized, double-blind, three-arm, placebo-controlled efficacy and safety study
    West RE, Biswas A, Rao R et al. · J Med Life · 2026RCT
  6. 06
    Dual impact of Ashwagandha: Significant cortisol reduction but no effects on perceived stress - A systematic review and meta-analysis
    Albalawi AA · Nutr Health · 2025SR
  7. 07
    Ashwagandha Does Not Enhance the Effect of High-Intensity Interval Training on Selected Energy Metabolism Parameters in Young Healthy Men
    Charmas M, Jówko E, Długołęcka B et al. · Nutrients · 2025RCT
  8. 08
    A New Ashwagandha Formulation (Zenroot™) Alleviates Stress and Anxiety Symptoms While Improving Mood and Sleep Quality: A Randomized, Double-Blind, Placebo-Controlled Clinical Study
    Mahadevan M, Gopukumar K, Gupta R et al. · Adv Ther · 2025RCT
  9. 09
    Amelioration and Immuno-modulation by Ashwagandha on Wi-fi Induced Oxidative Stress in Regulating Reproduction Via Estrogen Receptor Alpha in Male Japanese Quail
    Gupta V, Srivastava R · Reprod Sci · 2025
  10. 10
    Withania somnifera and Trigonella foenum-graecum as ingredients of testosterone-boosting supplements: Possible clinical implications
    Skrzypiec-Spring M, Pokrywka A, Kuliczkowska-Płaksej J et al. · Adv Clin Exp Med · 2025
  11. 11
    Ashwagandha (Withania somnifera (L.) dunal) root extract containing withanolide a alleviates depression-like behavior in mice by enhancing the brain-derived neurotrophic factor pathway under unexpected chronic mild stress
    Kim H, Choi HS, Han K et al. · J Ethnopharmacol · 2025
  12. 12
    Investigating the Impact of Ashwagandha and Meditation on Stress Induced Obesogenic Eating Behaviours
    Quinones D, Barrow M, Seidler K · J Am Nutr Assoc · 2025
  13. 13
    Nano-cubosomes of the phyto-active principle in Withania somnifera: LC-MS-NMR, anti-microbial, and insights of the anti-neuropathic and anti-inflammatory mechanism
    Raafat KM, Abdelwahab IA, El-Zahaby SA · Fitoterapia · 2024
  14. 14
    A Novel Herbal Composition Alleviates Functional Constipation, Reduces Gastrointestinal Transit Time, and Improves Bowel Function in Adults: A Double-Blind, Randomized Clinical Study
    Singh G, Dixit I, Kalman D et al. · J Am Nutr Assoc · 2024RCT
  15. 15
    Do "testosterone boosters" really increase serum total testosterone? A systematic review
    Morgado A, Tsampoukas G, Sokolakis I et al. · Int J Impot Res · 2024SR
  16. 16
    Nutraceuticals known to promote hair growth do not interfere with the inhibitory action of tamoxifen in MCF7, T47D and BT483 breast cancer cell lines
    Baker R, Dell'Acqua G, Richards A et al. · PLoS One · 2024
  17. 17
    Dietary restriction-induced alterations on estrogen receptor alpha expression in regulating fertility in male Coturnix coturnix japonica: Relevance of Withania somnifera in modulation of inflammation and oxidative stress in testis
    Baghel K, Azam Z, Srivastava R · Am J Reprod Immunol · 2024
  18. 18
    The Role of Dietary Ingredients in Mental Energy - A Scoping Review of Randomized Controlled Trials
    Nieman KM, Zhu Y, Tucker M et al. · J Am Nutr Assoc · 2024
  19. 19
    Withania somnifera ameliorates sexual arousal and impotence in stressed sexually sluggish male rats by modulating neurotransmitters and NO/cGMP/PDE5α pathway
    Yadav A, Mishra RK · J Ethnopharmacol · 2024
  20. 20
    Amelioration by Withania somnifera of neurobehavioural and immunological markers in time dependent sensitization induced post traumatic stress disorder in rats
    Rehman S, Faizan M, Ali NH et al. · Indian J Pharmacol · 2024
  21. 21
    SP-Max-A herbomineral formulation attenuates busulfan-induced oligospermia in mice by preventing loss of reproductive hormones
    Laddha AP, Joshi S, Kulkarni YA · Reprod Toxicol · 2024
  22. 22
    Phytoconstituents of Ashwagandha as potential inhibitors of human islet amyloid polypeptide (hIAPP): an in silico investigation
    Nandeshwar, Rout J, Panda SM et al. · J Biomol Struct Dyn · 2024
  23. 23
    Effects of Withania somnifera on Cortisol Levels in Stressed Human Subjects: A Systematic Review
    Della Porta M, Maier JA, Cazzola R · Nutrients · 2023SR
  24. 24
    Can Ashwagandha Benefit the Endocrine System?-A Review
    Wiciński M, Fajkiel-Madajczyk A, Kurant Z et al. · Int J Mol Sci · 2023
  25. 25
    A standardized Ashwagandha root extract alleviates stress, anxiety, and improves quality of life in healthy adults by modulating stress hormones: Results from a randomized, double-blind, placebo-controlled study
    Majeed M, Nagabhushanam K, Mundkur L · Medicine (Baltimore) · 2023RCT